How Does Temperature Affect Lipase Essay Example

How Does Temperature Affect Lipase Essay How does temperature affect the rate of reaction for Lipase? As the temperature increases, so will the rate of enzyme reaction. However, as the temperature exceeds the optimum the rate of reaction will decrease. I predict that at temperatures above 70 °C the enzyme lipase will become denatured and at temperatures below 10 °C the enzyme will become inactive. Since lipase operates within the human body I’d also predict that its optimum temperature would be around human body temperature which is approximately 37 °C. I predict that before the optimum temperature the rates will gradually increase and preceding the optimum there will be a drastic decrease in rate until the enzyme is denatured. I predict that the rate of enzyme activity at 45 °C will be half that of 30 °C. I predict that the rate of enzyme activity at 45 °C will be half that of 30 °C. Diagram courtesy of: http://www. rsc. org/Education/Teachers/Resources/cfb/enzymes. htm Diagram courtesy of: http://www. rsc. org/Education/Teachers/Resources/cfb/enzymes. htm In my controlled assessment I will be investigating the activity of lipase on milk fat at various temperatures so that I can then find an accurate temperature as to when the enzyme works at its optimum; when it becomes inactive and when it denatures. To find when the enzyme denatures is to find out when the bonds of this protein disintegrate and henceforth disable the enzyme from being of any further use. When these bonds break, the protein starts to unfold and loses some its properties. For example, a denatured protein usually becomes less soluble. As an enzyme, it will lose its ability to function as a catalyst. We will write a custom essay sample on How Does Temperature Affect Lipase specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on How Does Temperature Affect Lipase specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on How Does Temperature Affect Lipase specifically for you FOR ONLY $16.38 $13.9/page Hire Writer If the stress that is causing the denaturation continues, other changes may occur. Now that the normal structure of the protein is gone, new bonds may be formed, giving it a different shape. The bonds broken in a denatured enzyme is that of which links the polymers to form the amino acids. This means that if lipase were to denature at the higher temperatures it will then cause inactivity in breaking down the fat of the milk hence leaving the unchanged. In this investigation, however, there are numerous factors as to what can affect the investigations results. First of all, the temperature of the room can play a role in altering the results as it can change the temperature of both the solution and lipase. Moreover if one were to move the solution or lipase to another part of the room, or to carry out the investigation on a different day, the temperature surrounding the solution and lipase will change and henceforth change the temperature of the solution and lipase. Secondly, if the temperature of the water bath isn’t precisely the temperature it is supposed to be then, as expected, would change. Thirdly, the age of the contents can affect the concentration of the substrates which would then decrease the rate of reaction with lipase. Finally, there is the factor of human error, as we may not be capable of making perfect measurements consistently the amounts of each component will inevitably change, which would in effect change the results. Of this investigation our independent variable will be the rate of reaction, which we will measure by timing how long it would take for the solution to turn white after having the lipase poured in. Our dependent variable will be the time it takes for the solution to turn pink after having the lipase poured in. Our controlled variable is that of will be all other factors. Enzyme Diagram courtesy of http://students. cis. uab. edu/clight/finalprojectwhatisanenzyme. html Diagram courtesy of http://students. cis. uab. edu/clight/finalprojectwhatisanenzyme. html An enzyme is a molecule that changes the speed of reactions. Enzymes can build up or break down other molecules. The molecules they react with are called substrates; enzymes are catalysts. An enzyme works by allowing a substrate, or multiple substrates, to enter the active site, which is where the reaction takes place, and then to exit in either more or less pieces then it was when it first entered. The active site is unique to a specific substrate which means that other substrates cannot react with that enzyme unless the enzyme is modified. [An active site can be altered by a non-competitive enzyme which encircles the enzyme and alters the shape of the active site which could be very dangerous. ] Diagram courtesy of: http://www. wiley. com/college/boyer/0470003790/reviews/kinetics/kinetics_effec ors. htm Diagram courtesy of: http://www. wiley. com/college/boyer/0470003790/reviews/kinetics/kinetics_effectors. htm Note that the enzyme remains unchanged so that more of the some substrates can react. Note that the enzyme remains unchanged so that more of the some substrates can react. Structure Proteins are polymers made by joining up small molecules called amino acids. A mino acids and proteins are made mainly of the elements carbon, hydrogen, oxygen and nitrogen. Protein Protein Amino Acid Amino Acid Each gene acts as a code, or set of instructions, for making a particular protein. They tell the cell what to do, give its characteristics, and determine the way its body works. Each protein has a unique sequence of amino acids. This means that the number and order of amino acids is different for each type of protein. The proteins fold into different shapes. The different shapes and sequences give the proteins different functions, e. g. keratin are a fibrous protein found in hair and nails. If the gene has even the slightest of disorder within its sequence it could lead to an inaccurate order of amino acids and so a faulty protein or in our case faulty enzymes. Substrate concentration An enzyme has an active site where it binds the molecule (or molecules) it acts upon; the enzyme then catalyses a chemical reaction involving that molecule (or those molecules). That molecule (or those molecules) is called the enzymes substrate. So the substrate concentration is the concentration of the molecules an enzyme works on. Diagram courtesy of http://biochemistryquestions. wordpress. co m/2008/07/15/induced-fit-model-of-enzyme-substrate-interaction/ Diagram courtesy of http://biochemistryquestions. wordpress. o m/2008/07/15/induced-fit-model-of-enzyme-substrate-interaction/ In general, if there is an increase in substrate concentration, then more enzymes will be catalysing the chemical reaction and the overall rate of reaction will increase. It will continue to increase until all enzymes are actively binding substrate (called saturation), at which point no further increase in rate can occur, no matter how high you raise the substrate concentration. In my investigation into enzyme response to temperature this graph will be of relevant. Diagram courtesy of: http://www. sc. org/Education/Teachers/Resources/cfb/enzymes. htm Diagram courtesy of: http://www. rsc. org/Education/Teachers/Resources/cfb/enzymes. htm Denatured Denatured Denaturing Denaturing Less kinetic energy so the reaction slows down. Less kinetic energy so the reaction slows down. This graph illustrates the response that rate of enzyme activity has at various temperatures. At lower temperatures the rate is very low as there isn’t enough kinetic energy for the enzyme to work at its optimum, then you of course have the enzymes temperature optimum where the enzyme works best at. Finally you have the denaturing of the enzyme which eventually halts with the enzyme being completely denatured where it then will never have any activity. Collision Theory For a chemical reaction to occur, the reactant particles must collide. But collisions that do not have enough energy do not produce a reaction. The particles must have enough energy for the collision to be successful in producing a reaction. The rate of reaction depends on the rate of successful collisions between reactant particles. So the less successful collisions that occurs the less products created. Diagram courtesy of: ttp://www. worthington-biochem. com/introbiochem/tempeffects. html Diagram courtesy of: http://www. worthington-biochem. com/introbiochem/tempeffects. html The reason as to why particles may have or may not have enough energy to create products depends on the amount of kinetic energy in the particles. Hence why at lower temperatures the enzyme becomes inactive as there isn’t a high eno ugh temperature to create the necessary kinetic energy to create the products. As the temperature increases so does the rate which is due to more kinetic energy and hence more successful collisions. H An enzyme can also denature upon extreme pHs. with the extreme pH’s being 1 and 14, the enzyme would denature due to the hydrogen acids within the pH’s damaging the amino acid bonds within the enzyme. By damaging these bonds, the amino acids break apart, this in turn means that the enzyme’s active site will lose its shape, resulting in the denaturing of the enzyme. Henceforth, the optimum pH is in the middle of the pH spectrum as neutral pHs are unable to damage the bonds of the amino acids keeping the enzyme capable of reaction. Preliminary Method a. Get a test tube for each temperature being investigated. b. Add 5 drops, using a pipette, of phenolphthalein to the test tube. c. Measure out 5 cm3  of milk using a measuring cylinder and add this to the test tube. d. Measure out 7 cm3  of sodium carbonate solution using another measuring cylinder and add this to the test tube. The solution should be pink. e. Place a thermometer in the test tube. f. Place the test tube in a water bath and leave until the contents reach the same temperature as the water bath. g. Remove the thermometer from the test tube and replace it with a glass rod. h. Use the 2 cm3  pipette to measure out 1 cm3  of lipase from the beaker in the water bath for the temperature you are investigating. i. Add the lipase to the test tube and start the stopwatch. k. Stir the contents of the test tube until the solution loses its pink colour. l. Stop the clock/ watch and note the time in a suitable table of results. *A control was also investigated by having a test tube with the sodium carbonate, phenolphthalein and milk but without the lipase. This is to test as to whether the solution would turn from pink to white regardless of whether the enzyme was present or not. This was the original method which was used to carry out the preliminary investigation, however upon consideration it was decided that for the real practical a slightly alternate method should be used. In our edited method we made the changes of firstly, on putting the lipase into the water bath, this was because heating up the solution instead is to investigate the effects of the temperature of the solution as oppose to how the temperature of the enzyme effects. Secondly it was decided upon that we would not stir the contents for two reasons: firstly because by stirring the solution it spread the lipase around more which in effect speed the reaction up so much that it was impossible to time; secondly, by stirring the contents it often made the solution over flow which both made a untidiness and caused the volume of the contents to decrease. Finally it was decided that we were to limit the amount of temperatures being investigated as temperatures below 22? the enzyme was inactive hence taking too long to record the time it took for the solution to turn white, at temperatures over 55? c the enzyme, the lipase enzyme would be denaturing hence taking too long to be able to record as well. Final Method a. Get a test tube for each temperature being investigated. b. Add 5 drops, using a pipette, of phenolphthalein to the test tube. c. Measure out 5 cm3  of milk using a measuring cylinder and add this to the test tube. d. Measure out 7 cm3  of s odium carbonate solution using another measuring cylinder and add this to the test tube. The solution should be pink. e. Place a thermometer in the test tube. f. Place the test tube, containing only the lipase enzyme, in a water bath and leave until the contents reach the same temperature as the water bath. g. Remove the thermometer from the test tube. h. Use the 2 cm3  pipette to measure out 1 cm3  of lipase from the beaker in the water bath for the temperature you are investigating. i. Add the lipase to the test tube and start the stopwatch. k. Stop the clock/ watch and note the time in a suitable table of results. A control was also investigated by having a test tube with the sodium carbonate, phenolphthalein and milk but without the lipase. This is to test as to whether the solution would turn from pink to white regardless of whether the enzyme was present or not. Such changes were made in an attempt to improve the validity of the investigation. As is in the nature of an investigation it is impossible to make the results completely accurate and precise. What we c an do however is improve the reproducibility and reliability of our results by repeating the test multiple times. Risk Assessment Substance| Hazard| Risk| Risk rating*| Emergency action| Phenolphthalein | LOW HAZARD| Although it is not hazardous one should take precaution avoiding skin contamination. | 1| If in contact with eyes then flood eyes with water to wash it out. | Lipase| HAZARD| If in contact with skin it can cause an itch. If someone were to have an allergic reaction to lipase it could cause symptoms such as rashes. | 1| Seek emergency assistance if you believe you are having an allergic reaction to lipase. However wash it off as quickly as possible. Sodium Carbonate| IRRITANT| Sodium carbonate contributes to three major hazards: skin irritation, eye damage and internal effects. | 3| If swallowed, drink two or more glasses of water or milk. If in contact with skin use a cloth to wipe the sodium carbonate or rinse with water and if contact with eyes rinse thoroughly. | Milk| LOW HAZARD| If in contact with skin it can cause an itch, however some people may have an allergic reaction to t he substance. | 2| Acting in accordance to the severity of the reaction, one should wash it off as quickly as possible. Water| HAZARD| As the temperature of water we are to use will range between 10 °c-80 °c hot water may come in contact with us and burn ones skin. | 2| If hot water comes in contact with one’s skin one must rinse thoroughly with cold water to prevent further burning. | Test Tubes| HAZARD| If one were to drop a test tube, it would be very likely for it to smash, disintegrating over the floor which could then cut someone’s foot. | 2| If there is to be a broken test tube on the floor one must alert a member of staff and sweep the area whilst restricting anyone from crossing until one has finished clearing the area. Kettle| LOW HAZARD| If one were to knock a kettle over whilst boiling water the contents would spill and henceforth burn someone or something. | 1| Keep the kettle away from electrics and other peoples working areas. | *Risk rating out of / 5 Generic precautions As in all practical’s one must always take precaution of what is at hand, moreover it is obligatory to wear goggles to protect the eyes and to reduce the risk of skin contact one can wear disposable gloves. Another precaution to take is to ensure that no obstacles obstruct your movement as one may then spill a substance or break a piece of apparatus, a basic step is to push in all stools and to stand up when you do a practical. In addition a class should always leave their bags at the back of the classroom and put aside planners and books making a clear workstation. Any spills, accidents or injuries should be dealt with immediately and inform a member of staff. Review of Evidence The shape of the graph resembles that of the rate of enzyme activity graph on page 3, an arc. With the shape of the graph being similar to an arc, it displays clearly that there is a definite optimum to the rate of lipase’s activity and the stages of inactivity and denaturing. The optimum temperature of lipase on this rate graph was the same in both my preliminary data and my real results data which was 30 °c and in both instances the shapes of the graphs do resemble that of an arc. In the preliminary graph, the range bars were rather extensive for example, at 35 °c the difference between the highest (non-anomaly) result and the lowest was 0. 13 in rate. These inaccurate results could have been due to multiple factors with the more obvious being either human error or faulty equipment. By having such a difference in results it only justified the changes which we had made for the real investigation. When looking back upon my original hypothesis, it stated that before the optimum temperature the rates would gradually increase due to the lack of kinetic energy provided from the heat. Upon reviewing the graph it is clearly illustrated that there is an increase in rate from temperatures 22 °c-30 °c with an increase of 0. 26 in rate. I also predicted that the optimum temperature would be 37 °c, due to the fact that lipase operates in the human body and the human body’s temperature should be 37 °c. By analysing the evidence of which the graph presents it tells me that the highest rate of reaction was that of 30 °c, meaning this was the optimum temperature. Finally, I predicted that once the optimum was exceeded, the rates wo uld begin to decrease as they cannot function at such temperatures due to the breaking in the peptide bonds that holds the amino acids together. Once this bond is broken, the enzyme is reduced to its primary structure which is just peptide bonds occurring – the functional structure of the enzyme is lost and it is no longer functional; denatured. After the optimum temperature, which was 30 °c, the rate of reaction began to decline as the temperatures increased. Henceforth, my prediction was right in saying that once the optimum temperature had been passed; the denaturing process would begin to take place, meaning the rates of reactions would become slower. Upon looking back at my quantitative prediction, which stated that â€Å"at 45 °c will be half that of 30 °c. † However, the decrease in rate was far more drastic then I had predicted. (Rate of 30 °c was 0. 032; rate of 45 °c was 0. 005. ) This means that the process of denaturing was far quicker than I had previously predicted which in turn means that my quantitative was incorrect. However, if I were to replace the 45 °c figure in my initial quantitative prediction with 35 °c it could then be plausible as the rate of 35 °c was 0. 011 (30 °c-0. 032. )In addition, I would further modify my initial prediction by Secondary data By analysing the provided secondary data I shall be able to further prove or disprove the evidence that I had recorded. By being able to prove my data with secondary data which has the same outcome and conclusion it proves that that the data is repeatable as there are externally recorded results that support the results that I had recorded. Figure 4 courtesy of: http://www. currentscience. info/upload/IssuesFile/29_issues_Article%2010. pdf Figure 4 courtesy of: http://www. currentscience. info/upload/IssuesFile/29_issues_Article%2010. pdf Figure 3 courtesy of: http://www. google. co. uk/url? sa=t;rct=j;q=;esrc=s;source=web;cd=7;ved=0CGIQFjAG;url=http%3A%2F%2Fwww. diagnosisp. com%2Fdp%2Fjournals%2Fview_pdf. php%3Fjournal_id%3D1%26archive%3D0%26issue_id%3D31%26article_id%3D1135;ei=nrjEUJ2XC8HJ0AXPy4DACQ;usg=AFQjCNEb15WjPAyJMMgCDAjs3ZaorsN3qg;sig2=mf7h7XRNBjWBD3cdMS2v-w Figure 3 courtesy of: http://www. google. co. uk/url? sa=t;rct=j;q=;esrc=s;source=web;cd=7;ved=0CGIQFjAG;url=http%3A%2F%2Fwww. diagnosisp. com%2Fdp%2Fjournals%2Fview_pdf. hp%3Fjournal_id%3D1%26archive%3D0%26issue_id%3D31%26article_id%3D1135;ei=nrjEUJ2XC8HJ0AXPy4DACQ;usg=AFQjCNEb15WjPAyJMMgCDAjs3ZaorsN3qg;sig2=mf7h7XRNBjWBD3cdMS2v-w Figure 1 (left) ; 2 (above) courtesy of: http://www. slideshare. net/wkkok1957/effect-of-temperature-on-lipase-activity-using-ph-sensor Figure 1 (left) ; 2 (above) courtesy of: http://www. slideshare. net/wkkok1957/effect-of-temperature-on-lipase-activity-using-ph-sensor Comparing the data sets As is clearly shown in all of the above figures there is a clear optimum. In terms of the optimum temperature, it ranges from 35 °c (figure 2 ; 3) to 25 °c (figure 4. Whereas in the recorded data that I had collated, it was 30 °c with the rate for 35 °c being significantly less than half of the rate for 30 °c. When comparing the rates at 20-25 °c another difference in rate had occurred as you can see in figures 1, 2, 3 ; 4 there isn’t such a sharp increase in rates whereas in my own results there is a steep increase in rate between 22 °c and 30 °c, a difference of 0. 026. ) Moreover, in terms of the temperature at which the lipase denatures also varied as the denatured point in figure 3 is at 50 °c whereas the temperature at which the lipase denatured in my investigation was at 55 °c. Finally in terms of the shape of the graphs you can see that in figure 2 the shape of the graph is of a rather steady contour oppose to the sharp point that is of my graph shape. The foremost reason as to what caused such differences was the fact that the secondary investigations used an alternate for example in figures 1 ; 2 the method utilised was slightly different as they used more accurate pieces of apparatus for example they used a micropipette to measure the sodium carbonate into the test tube which would ensure for far more accurate measurements then I had made. Secondly they used a pH probe a Logger Pro to detect the change in the milk which would also prove for much more accurate readings in comparison to detecting the change with the eye as we cannot see the entire of the solution and we, henceforth, could record a shorter or longer time to the actual figure as we would essentially be guessing as oppose to knowing when the reaction was definitely complete. On the contrary however, they only repeated each temperature 3 times so as to collect triplicate data. In conclusion I would say that by analysing secondary data it does support my data in its general trend but in terms of individual figures, inactivity and denatured points I am unable to defend and justify that my investigation is completely reproducible. I must say that in all, I would say that the reason as to why there is a difference in the primary data and secondary data is due to multiple factors such as alternate methods, alternate apparatus and an alternate working environment. However, in total, I do feel confident in saying that my results are reproducible to such an extent that it can resemble that of the actual figures and graph shape. Evaluation of errors I believe that the changes made to the preliminary method for the real investigation did improve the overall accuracy of the data in the real results data. However, in the results there were many outliers that were recorded, six in total. These errors and possible inaccuracies were made possible by such factors as human error, equipment error and technique rror. In terms of human error we may have made the mistake of timing the reaction wrong because the people who are timing the investigation may time it wrong. Secondly, there may be a difference in opinion in when the reaction would have fully completed as one may say that the solution still contains traces of pink yet another may say that the solution has no traces left. Finally, there could have been the human error of inaccurately measuring the p ortions of the solution. In terms of equipment error, sometimes the water baths were unable to heat the solution to the specified temperature of which were trying to investigate which would then have the effect of us collating alternate data to what we should have got, this would then alter our rate bars as they be higher or lower. Furthermore, there may no longer have been a real difference in the data’s even if there was supposed to be. Secondly, our portions of the solution may have been measured inaccurately as the measuring cylinders used may have not been accurate enough for us to get precise measurements. On top of this, whilst using the pipette to measure the contents into the measuring cylinder, air bubbles were created which then alter our results as we would then be measuring a different quantity as opposed to the proposed temperature. Finally such technique errors occurred such as the lipase may have not spread equally amongst the solution which would have left a section of the solution untouched by the enzyme. Furthermore as we took the lipase out of the water bath the temperature of the lipase would either increase or decrease if above or below the room temperature. To improve the accuracies and reliabilities of the data collected and to reduce the errors as is mentioned above I would make such alterations to the existing method: -To ensure that the lipase truly got to the temperature that it was supposed to be at an improvement would be as to set the temperature of each water bath 3 °c higher than what was prepared for which would make it easier for the lipase to heat up to the specified temperature. To increase the accuracy and eliminate the of measuring incorrectly the solution ingredients an improvement could be to use a syringe as oppose to a pipette as the pipette can’t measure as accurately as a syringe because whilst using the pipette bubbles where constantly created which made it incredibly difficult to then accurately measure the contents that were to be measured in. -As is the nature of foods and drinks the milk would eventually surpass the date hat it was meant to be consumed by. However this means that the bacteria within t he milk may function in a different manner because the bacteria uses the lactose sugars to reproduce, they change it from â€Å"lactose sugar† into â€Å"lactose acid,† which tastes sour and it becomes a huge food borne illness risk to consume it and it must be discarded. Instead then we can use such alternatives as UHT or powdered milk as they have longer life spans because more of the bacteria is removed. To remove the factor of misjudgement whilst trying to detect as to whether the solution has lost all traces of pink an improvement can be to use a pH probe next time as the pH probe could then accurately detect once the reaction has completely finished by seeing when the figures stop changing on the pH probe. Improved Method a. Get a test tube for each temperature being investigated. b. Add 5 drops, using a pipette, of phenolphthalein to the test tube. c. Measure out 5 cm3  of milk using a measuring cylinder and add this to the test tube. . Measure out 7 cm3  of sodium carbonate solution using another measuring cylinder and add this to the test tube. The solution should be pink. e. Place a thermometer in the test tube. f. Place the test tube in a water bath and leave until the contents reach the same temperature as the water bath. g. Remove the thermometer from the test tube and replace it with a glass rod. h. Use the 3 cm3  syringe to measure out 1 cm3  of lipase from the beaker in the water bath for the temperature you are investigating. . Add the lipase to the test tube and start the stopwatch. k. Using the pH metre wait until it displays that no pink resides in the solution. l. Stop the clock/ watch and note the time in a suitable table of results. *A control was also investigated by having a test tube with the sodium carbonate, phenolphthalein and milk but without the lipase. This is to test as to whether the solution would turn from pink to white regardless of whether the enzyme was present or not. Evaluation of procedures When analysing and evaluating the procedures I shall divide the section into four sectors: precision, accuracy, repeatability and reproducibility. Precision refers to how well experimental data and values agree with each other in multiple tests. [1] The only evidence to demonstrate the precision of the data is the range bars. All range bars excluding 30 °c ;0. 001, however for 30 °c the range is ;0. 001. This proves that the precision of the data was quite good with the exception of the data for 30 °c. By having a small range in data it exemplifies precision of the data as they are all within a similar region of figures. However with 30 °c the data was rather spread meaning that the results for 30 °c degrees were not precise due to the fact that my range bar is rather spread when compared to the likes of the data from 22 °c where the range bar is a quarter of the size of the range bar for 30 °c. This provides me with the necessary evidence to believe that the rest of my results were precise, with the results for 30 °c being the exception. The ability to obtain consistent results when measuring the same part with the same measuring instrument. [2] Upon considering the repeatability of this investigation one can say that the results are most certainly repeatable as the data resembles that of which others have collated and that of the preliminary data. If one were to repeat the investigation with the improved method then the investigation is, with no doubt, repeatable as the evidence lies within the secondary data that supports the data of which I have collated. Accuracy refers to the correctness of a single measurement. Accuracy is determined by comparing the measurement against the true or accepted value. [3] Although there is nothing we can do to improve the accuracy per say, we can, for example, remove outliers that do not share any resemblance to that of the true value, we are able to make more accurate calculations as to what the average is because we are taking out a value that does not mean anything to the true value. By doing so in my calculations it not only improved the accuracy of the results but it also exemplified how some factors could change the results so drastically. This demonstrates that although we can control most factors that alters the results we can’t completely control them as there are endless factors as to what can affect the results recorded, for example the room temperature could affect the results is could have heated or cooled the solution. By controlling the variables of which were possible to control we did all that was possible for us to do in order of making the investigation valid. Furthermore, by repeating the outliers again to get a new set of results it would provide for a more accurate average. This is something that was not done due to the lack of time Reproducibility is one of the main principles of the scientific method, and refers to the ability of a test or experiment to be accurately reproduced, or replicated, by someone else working independently. [4] If the results were to be reproducible then it would be possible to look at secondary data and see that it closely resembles that of the results I have provided. When comparing my results to that of peers of who are carrying at the same investigation there is most certainly a resemblance in the overall shape of the graph. Although the rates may differ the general trend of the graph does suggest the same conclusion that there is a definite optimum at around 30 °c-35 °c. http://www. slideshare. net/wkkok1957/effect-of-temperature-on-lipase-activity-using-ph-sensor -this is a link to someone else’s investigation and results (Tony Hong), from this link you are able to see Tony’s investigation and results that follow a similar method as to mine. With this it is possible to see the results and henceforth make

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Persuasive message - Essay Example I was aware that some of the stockholders were not receptive to my ideas, and some of the officials did not want me to be promoted. I made it a point to mention the names of those whom I perceived to be hostile in a patronizing sound, as if the ideas injected in my project were suggested by them. I was aware they had a high level of interest, I was able to cut to the chase quickly, and went directly to the key messages (na 2004). Object of Persuasion: The product we were trying to sell is a residential property under the real estate industry. The competition with the other real estate companies for the kind of market we wanted to monopolize was quite stiff, particularly in the area where I will be transferred, which was not performing well at that time. I proposed to lower the selling price, and to allocate additional budget for special incentives for the Account Consultants, a kind of motivation that is badly needed in that area. Argument: I presented a graph, that depicted the tren ds in the sales productivity when there were promotions and when no promotions were offered to the customers. I had to defend my proposal because it entailed additional cost on the part of the company, which they were not willing to accede to at the start.

Principles of Statistics In-Module Assessment Coursework

Principles of Statistics In-Module Assessment - Coursework Example The period over which the exchange rate is observed is January, 1973 to January 2013. Annual observations are used; for each year, a single value of the exchange rate is observed. Thus, there are 43 annual observations on the exchange rate in the sample of data. Before turning to the specific characteristics of the series, it may be useful to note the significance of forecasting foreign exchange rates. For any economy that is open to international trade, the foreign exchange rates play important roles in deciding policies governing most of the important macro-economic variables in the economy. Particularly since the settlement of most international transactions occur within a short future horizon, the exchange rates likely to be applicable at the time of settlement assumes importance. The magnitude of the associated cash flows in terms of the domestic currency are evaluated by using foreign currency conversions. And these conversions are obtained by denominating the domestic currency by the foreign currency of the trade partner through the foreign exchange rate for the pair of countries (Taylor 1995). ... Since the natural logarithm is a monotonic transformation, all dynamic properties of the series are preserved, only the scale of the magnitude is smaller. Figure 1: The exchange rate between the Japanese Yen and the US Dollar The first and foremost point to be noted is that the price of the Dollar has exhibited a downward trend over the time horizon under consideration. Starting at slightly above 5.8 in 1971, the value of the series has ended at around 4.6 in 2013. However, the series has not continuously declined. It has exhibited periods of upward movements as well as downward movements. The length and magnitude of the downward movements however, dominate the trend. As a result, the overall trend of the series is that of a decline. A closer look reveals that the series exhibits a particular persistence: a downward movement continues for a few years as does an upward movement. That is, once a dip starts, it persists for a few years. Similarly, once an upward swing starts, it seems t o persist for a few years. This pattern of persistence will become important in the forecasting phase. It should also be noted that the average length of the persistence of upswings is visibly smaller than the average length of the downswings. This pattern is particularly clear for the years after 1985. The other pattern worthy of note is that the overall magnitude of increases in the exchange rate over periods of appreciation is smaller than the observed magnitudes of decline in the exchange rate during periods of decreases. Barring the years between 1977 and 1983, this holds true for every period of decline and period of increase. Therefore, the observed pattern of decline in the Japanese price of the US dollar is explained by two

Biodiversity Lab Essay Example for Free

Biodiversity Lab Essay 1. Explain how the ecosystem was affected by the missing species for each round of the demonstration. a. Round 1 = white bead representing lichens. Our laboratory reading for the experiment stated that lichens are instrumental in the development and needs for all species. The text stated, â€Å"Lichens play a part in the creation of soils from which plants can obtain nutrients. Like all living organisms, lichens need nutrients and energy to grow. Nutrients may be obtained from the air including dust, water, and from the substrate organisms grows on obtaining energy through photosynthesis, which is the role of the algal partner. They may also be incidentally fertilized hybrid and insect dung† (Bottcher Rex, 2012, p. 43). With the information obtained from the reading, without lichens, bees, trees, flowers, and humans will all have negative repercussions. b. Round 2 = Yellow bead, representing bees. No bees and lichens the trees will not be pollenated, which will result in a loss of a food source  for humans since bees pollenate trees and flowers for food. Without bees to pollenating, the ecosystems takes another dramatic step towards extinction. One study stated, â€Å"Deduction from floral structure indicates that the flowers of many crops and wild plants are adapted for bee pollination. Further experimental studies of pollination are much needed, to see whether inadequate pollination limits seed set, and which insect species are potential or actual pollinators. The adequacy of pollination of a given plant species will vary regionally with climate, habitat and the nature and density of the local pollinator community† (Corbet, Williams, Osborne, 1991, para. 3). Minus the bees, the results for all other species will be negatively impacted. c. Round 3 = Orange bead, representing trees. The trees are dependent on lichens and with lichens removed. The trees will not have enriched soil to keep living. This is the reason for loss of oxygen for all species who require it and without bees, the fruit that some trees produce will cease to exist, causing food shortages and lack of shade and protection for flowers and humans (Bottcher Rex, 2012). d. Round 4 = Red bead, representing flowers. Lichens removed the soil could not adequately support nutrients required to keep alive. The flowers needs protection and shade from trees to sustain life and the trees were removed due to soil composition and loss of lichens from ecosystem. 2. Provide one action we as humans engage in that leads to the extinction of each of these components. Action affecting Lichens = The use of pesticides, chemicals, other pollutants, and people cause damage to this component that can lead to the irreversible impact on the lichens. Action affecting Trees = The destruction of soil and pollution, a major area where humans are impacting trees is through the removal of forests for trees to support human projects and constructions. â€Å"Ninety-three percent of the forestland in the United States is Privately owned† (Hazel baker, 2008, para. 1). Action affecting Flowers = Even though our chemicals are killing these flowers and making the soil contaminated and resistant to flower species. The use of pesticides and other chemicals, flowers are not engineered like crop seed to survive these chemicals. Action affecting Bees = Due to several human factors, bees are being threatened and destruction of their habitat is the number one reason. For a habitat to remain successful and sustain populations it must provide nesting sites and a seasonal succession of forage, and humans are depleting these areas through population growth and industrialization (Corbet, Williams, Osborne, 1991). 3. Provide three specific actions that humans can take to minimize our impact on the ecosystem and ensure the survival of lichens, trees, flowers, and bees. Action 1 = Bees are a critical part of the ecosystem. Humans need to ensure habitat sustainment to sustain bee populations do not deteriorate to levels that cannot be corrected. â€Å"Bees need food (nectar, pollen) provided by insect-pollinated plants in order to reproduce and produce more bees† (Corbet, Williams, Osborne, 1991, p 2). By creating hives and environments that are conducive to bee sustainment, it is possible that strides can be to reverse the downward trend in populations. This is critical as one study showed. It stated that the loss of bees was a domino effect to other species. It stated, â€Å"If bee pollinated flowers  do not seed, the integrity of Europe’s remaining semi natural vegetation will be destroyed and the colorful flowers of the countryside will be lost. This in turn will deprive many other herbivorous or seed-eating insects, birds and small mammals of their host plants and/or food,  with consequent further loss of species diversity†(Corbet, Williams, Osborne,1991, p 2). Through the saving of the bees, the increase of pollinated of other species helps ensure survival of flowers Action 2 = Humans can stop polluting the soil and surface of lands through illegal dumping, spills, etc. that contaminate and kill lichens that are critical in soil creation and providing nutrients to other plants and trees. With the help of government agencies and through stricter enforcement, creation of neutral pesticides and chemicals, and other products to eliminate harm to the environment and providing a healthier ecosystem for all life to thrive. Action 3 = The best way to save our tree populations is to protect the public lands from forest harvesting and ensure there environmental regulations for commercial logging businesses. â€Å"93% of the forestland in the United States is privately owned† (Hazelbaker, 2008, para. 1). That means that there are plenty of lands available for harvesting and ensuring forests do not die off and leave the negative impacts and loss of a precious resource. Due to the benefits that can be found in the national and state forests, such as clean air, fresh water, etc. I feel it is a good practice to prevent commercial access on these lands. These lands serve as a refuge to wildlife and often provide unique opportunities to the citizens who rarely get an opportunity to experience nature and these environments. POST LAB QUESTIONS 1. Develop a hypothesis on which pot you believe will contain the highest biodiversity. Hypothesis = One pot has direct exposure to sunlight it will have the highest level of biodiversity 2. Based on the results of your experiment, would you reject or accept the hypothesis that you produced in question 1? Explain how you determined this. Accept/Reject = Reject. Table 2 of this experiment, the pot that was placed from all windows had a greater diversity of growth from the random seeds planted in it, versus the one with direct sunlight. The pot that had direct sunlight yielded smaller totals in each of the five plants planted in each pot as well as a smaller overall plant yield. While some individual plants had close results, there was a total difference of the total plants of 48. 3. If each pot was a sample you found in a group of wildflowers, would you determine based on the diversity of flowers that the ecosystem is healthy? Why or why not. Answer = I would say that each sample would yield results of a healthy ecosystem based on the diversity in each sample. Each plant has samples of  all five seeded plants planted and yielded results in two weeks. While both specimens yielded results, I would say the ecosystem sample that was not in direct sunlight did show signs of a healthier ecosystem and believe that the temperature and light changes the other sample was exposed to could have played a part in the results. Example, living in Arizona my residence has low humidity and low temperatures at night and moderate temperatures in the mid-morning through sundown that affected the sample with sunlight exposure. The other sample, the one away from the windows, sat in the middle of my apartment on a shelf in the laundry room, with no direct exposure to sunlight, but not completely in the dark, had more stable temperatures and had constant ambient light exposure through the day and did not suffer cold drafts from the windows that could have made the other specimens plants yield lower results. 4. How does biodiversity contribute to the overall health of an ecosystem? Provide specific examples and utilize at least one scholarly resource to back your answer. Answer = Biodiversity contributes to all levels of an ecosystem. â€Å"Biodiversity, short for biological diversity, includes the genetic variation between all organisms, species, and populations, and all of their complex communities and ecosystems. It also reflects to the interrelatedness of genes, species, and ecosystems and their interactions with the environment† (Bottcher Rex, 2012, p. 39). The experiment with the two pots is similar to that of a forest ecosystem. â€Å"A functioning forest ecosystem, for example, exhibits rates of plant production, carbon storage, and nutrient cycling that are characteristic of most forests† (Chapin, Costanza, Ehrlich, Golley, Hooper, Lawton, Tilman, 1999, para. 9). The five seeds that grew in each pot were sharing nutrients from the soil and the evidence showed that certain species thrived in numbers over the others that grew. As one research analysis on biodiversity, there are always species interactions that have impact on the other species. The study stated, â€Å"Ecosystem functioning results from interactions among and within different levels of the biota, which ecologists describe as a nested hierarchy. Example, green  plant production on land is the end product of interactions of individual plants nested within populations; interactions among populations nested within a single species; interactions among a variety of species nested within a group of functionally similar species; and so on up to the level of interactions between different types of ecosystems nested within landscapes† (Chapin, Costanza, Ehrlich, Golley, Hooper, Lawton, Tilman, 1999, para. 11). While some ecosystems continue to exist, some thrive, the harm being done to, and overall health of the ecosystem is dependent on the actions we as humans take. Our society has destroyed ecosystems, species, etc. and pollution, air quality, and other factors have seen many ecosystems destroyed and become extinct. â€Å"From current research, we have identified the following impacts on ecosystem functioning that often result from loss of biodiversity: -Plant production may decline as regional and local diversity declines; -Ecosystem resistance to environmental perturbations, such as drought, may be lessened as biodiversity is reduced. -Ecosystem processes such as soil nitrogen levels, water use, plant productivity, and pest and disease cycles may become more variable as diversity declines† (Chapin, Costanza, Ehrlich, Golley, Hooper, Lawton, Tilman, 1999, para. 11). In the end, if action is not taken soon, the devastation created through our industrialization, over population in countries across the world, etc. can lead to consequences that can see more devastation and harm that may not be able to be undone if our society fails to take the necessary action to sustain life of all ecosystems. References Hilmo, O., Sà ¥stad, S. M. (2001). Colonization of old-forest lichens in a young and an old boreal i Picea abies forest: an experimental approach. Biological Conservation, 102 (3), 251-259. Chapin III, F. S., Costanza, R., Ehrlich, P. R., Golley, F. B., Hooper, D. U., Lawton, J. H., Tilman, D. (1999). Biodiversity and ecosystem functioning: maintaining natural life support processes. Washington, DC: Ecological Society of America. Hazelbaker, J. (2008). Logging on national forests national forest management zero cut – no. Retrieved from: http://www.worldissues360.com/index.php/logging-on-national-forests-national-forest-management-zero-cut-no-26744/. Corbet, S. A., Williams, I. H., Osborne, J. L. (1991). Bees and the pollination of crops and wild flowers in the European Community. Bee World 7 (2), 47-59.

Women in The Birthmark Essay -- Birthmark Essays

â€Å"The Birthmark† – Women  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚        Ã‚  Ã‚   â€Å"Everything he has to say is related, finally, to ‘that inward sphere.’ For the heart is the meeting-place of all the forces – spiritual and physical, light and dark, that compete for dominance in man’s nature. . . .† (McPherson 68-69). McPherson’s â€Å"heart† is the key to understanding the role of women in Nathaniel Hawthorne’s tale, â€Å"The Birthmark.†    Only imperfection is what nearsighted Aylmer sees in the birthmark on Georgiana’s cheek. But he is unfortunately oblivious to   the virtue in her soul, the deep beauty contained in the depth of her love for him. The wife’s virtue leads her onward and upward; the husband’s lack thereof and inability to appreciate virtue in his Georgiana leads him downward and downward.    The concept of women is established in the very opening paragraph of â€Å"The Birthmark.† The narrator introduces Aylmer as a scientist who found â€Å"a spiritual affinity more attractive than any chemical one,† referring to his love for Georgiana. She is portrayed as having meaning in Aylmer’s life – not in first place, but in second place to his scientific interests.    Even after Aylmer has â€Å"persuaded a beautiful woman to become his wife,† he is not capable of loving her properly, unselfishly, because he â€Å"had devoted himself, however, too unreservedly to scientific studies ever to be weaned from them by any second passion.† The narrator seeks to justify this error or lack in Aylmer by explaining that â€Å"it was not unusual for the love of science to rival the love of woman in its depth and absorbing energy.† Already at the outset of the tale, the reader perceives that Georgiana is going to be shortchanged in this marriage. She is exposed to the problem initial... ...el . The Birthmark Electronic Text Center, University of Virginia Library http://etext.lib.virginia.edu/etcbin/toccer-new2?id=HawBirt.sgm&images=images/modeng&data=/texts/english/modeng/parsed&tag=public&part=1&division=div1    McPherson, Hugo. â€Å"Hawthorne’s Use of Mythology.† In Readings on Nathaniel Hawthorne, edited by Clarice Swisher. San Diego, CA: Greenhaven Press, 1996.    Stewart, Randall. â€Å"Hawthorne’s Female Characters.† In Readings on Nathaniel Hawthorne, edited by Clarice Swisher. San Diego, CA: Greenhaven Press, 1996.    Swisher, Clarice. â€Å"Nathaniel Hawthorne: a Biography.† In Readings on Nathaniel Hawthorne, edited by Clarice Swisher. San Diego, CA: Greenhaven Press, 1996.    Williams, Stanley T. â€Å"Hawthorne’s Puritan Mind.† In Readings on Nathaniel Hawthorne, edited by Clarice Swisher. San Diego, CA: Greenhaven Press, 1996.

Globalisation Drivers

Explain what is meant by the term globalisation. Identify and analyse the key drivers of the process of globalisation over the last twenty years. During the mid 1990’s the International Monetary Fund has defined globalisation as: ‘The growing interdependence of countries world-wide through the increasing volume and variety of cross-border transactions in goods and services and of international capital flows, and also through the more rapid and widespread diffusion of technology’ (Turner, 2006).Over the years, this interdependence of countries worldwide has increased dramatically. An indication of this has been the increase in the number of domestic and foreign strategic alliances by six times during the period 1989-1999 (Nam-Hoon Kang, Organisation for Economic Co-operation and Development, 2001). This change clearly indicates how companies from all over the world interact with each other and form partnerships in response to the phenomenon of globalization.The mai n drivers that have helped globalization to expand and deepen over the past years have been technological revolutions such as the widespread use of Internet and the ease of trans-boundary travelling, the creation of international institutions that encourage free trade by removing trade barriers, the establishment of multinational corporations which seek to increase their profits by taking advantage of what globalisation has to offer (Economics for business 5th edition John Sloman p. 498) and last but not least the change of governments’ policies towards deregulation and privatisation (Development in Practice Taylor & Francis p. 24). This study aims to outline and examine these key drivers that made it possible for globalisation to evolve. The study will also focus on the magnitude of the drivers with relation to globalisation. â€Å"Globalisation is both a result and a force of modernisation and capitalist expansion, entailing the integration of all economic activity (local, national, and regional) into a ‘global' market place: that is, a market place that transcends geopolitical borders and is not subject to regulation by nation states. (Development in Practice Taylor & Francis p. 524) Technological revolutions are viewed by many as one of the key drivers of globalisation. (Bradley 1993, Dicken 1992). Firstly, the reduction in transportation costs and the ability to communicate freely and easily due to the advances in technology have caused tourism to rapidly grow over the years (Tourism in the age of globalisation Salah Wahab, Chris Cooper p. 320). Most importantly, not only individual people as passengers were benefited by the lower transportation costs.In the last half of the twentieth century, the price of transporting products worldwide has fallen dramatically due drops in the cost of air travel, the containerization and increasing ship size (Chrystal, 2007, p. 11). Containers have the ability to move non-fragile goods at the cost of 1% of retail value to any place. In the past years, when the transportation of goods was done through shipping before containers were invented, the cost involved used to be around 10%-20% of retail value. by BCRA) The striking difference in cost indicates the new potential for transfer of goods at increased quantities at a faster and cheaper rate from one place to another. In addition to this, the rapid grow of communications, especially through Internet has added significant strengths to globalization (Economics of globalisation By Partha Gangopadhyay, Manas Chatterji). The Internet has provided a powerful and cheap tool for sharing of information on goods and services through the form of advertising. This helped firms to boost their sales since they can target a bigger range of potential customers at a very low cost.The trends clearly show a constant increase of Europeans e-commerce sales from 2006 to 2011, reflecting a similar increase in the corporations that are investing in the e-bu siness (ELECTRONIC COMMERCE AND THE GLOBALISATION ERA). Furthermore, internet enables free, fast and easy communication of ordinary people from all around the world which also serves as a factor that promotes globalisation and exchange of views and ideas on new products and services (Economics of globalisation By Partha Gangopadhyay, Manas Chatterji).In the recent years, technology is undoubtedly an enabling driver of globalisation but the latter is also influenced by economic motives. This actually means that globalisation is also driven by the economic motives of people. These motives have to do with shifting patterns of production and consumption from one place in the world to another (Tourism in the age of globalisation p. 321). More specifically, businesses seek new opportunities to lower their costs, to achieve savings of scale and to establish a competitive global position by gaining a greater global market share (Economics for business 5th edition).As a result of these motiv es, the foreign direct investment over the years has increased substantially and inevitably the number of cross-borders mergers and acquisitions has also increased. Corporations are trying to lower production costs by shifting the production to countries with cheap labour as well as to countries with abundant resources such as raw materials. China, India and Eastern Europe have been in the recent years the targets of FDI since they possess a production comparative advantage because of abundant and low wage labour force (By Great Britain: Parliament: House of Commons: Treasury Committee).Furthermore, the reduction in trade barriers, the lower transportation costs and the development of stock markets, increased the corporations’ ability to trade and invest at a global scale. For example, stock markets have served as mechanisms for important inflow of capital for corporations since they can go public and raise significant capital that can be used for FDI (http://www. globaldesig nandbusiness. org). Someone can easily distinguish that while benefiting from what globalisation is offering the corporations are themselves driving the phenomenon through their innovations on technology and their overseas activities.The corporations’ economic activities, which are mentioned in the previous paragraph, are also related to the trade institutions that are world widely formed. The world trade institutions are associations that aim to the liberalisation of trade and encouragement of transnational economic actions. Such institutions are namely the WTO, NAFTAA and EFTA. These institutions have similar goals ie to increase competition and economic efficiency through the reduction of trade barriers and the governments’ regulations over trade within the members of their organisations.These organisations are having their members agreeing in reduction of tariffs and other policies (such as antidumping and subsidies) in order to make the governments transparent. Th e organisations are also setting special committees with the task of making sure that the pre-agreed policies are followed and that no member gets out of line. Because of the establishment of such institutions and the clear increase in their membership with ascending time the world trade has increased dramatically (http://www. wto. org, http://www. nafta-sec-alena. org).A good indication of that is the fact that the United Kingdom’s imports and exports contribution to its GDP has increased from 45% to 57% over the period of 1950 to 2005 ( Great Britain: Parliament: House of Commons: Treasury Committee). Consequently, as the world institutions become stronger more active and more influential, globalisation is strengthened due to the improved and amplified interactions of corporations and countries as a whole. The governments as the years pass are trying to create a more competitive environment so that they can become attractive for foreign inflows of capital and investment.Fur thermore, they want to minimize the outflows to less regulated countries (Global public policy: governing without government: Wolfgang H. Reinicke p. 15). In order to achieve these goals, they have been converting their policies towards a more economically favourable manner. Inevitably they have been trying to reduce their trade barriers and accelerate economic growth by joining the world trade institutions (e. g. WTO) as well as the free trade blocks such as the E. U and ASEAN. The trading blocs have a large number of objectives that bring the country-members closer to each other.Some of these objectives demand that the members cannot practise anticompetitive behaviour such as high tariffs, quotas and anti-competitive taxation whereas additionally peace, mutual respect and cultural acknowledgement are also promoted (http://www. aseansec. org, http://europa. eu). Looking at another aspect in the chapter of the governments’ policies that is driving globalisation, someone could identify the remarkable example of the transition countries. The transition countries are suggested to be China, the former Soviet Union, ex-communist Europe countries and third world countries such as India (http://en. ikipedia. org). Over the past 20 years these countries have undergone various economic and political changes that have substantially shifted the countries from the previously central planned economies towards free market economies. As a result liberalisation and privatisation have been encouraged (Privatization in transition countries: By Oleh Havrylyshyn, Donal McGettigan p. 7 and 8). Because of those changes, globalisation has been favoured through its inherent characteristics to become stronger and deeper with modernisation and capitalist expansion. (Taylor &Francis). Looking back to the key drivers of globalisation we have presented in this study, they have all been relatively important since they are all favouring globalisation correspondingly. It is clear that the technological advances and the governments’ policies and behaviours separately and distinctly are really adding to the increasing pace of globalisation. However, the interesting thing that someone could safely say, it is that these drivers are interrelated which suggests that one driver is driving the other and both of them are driving globalisation. References: 1.European Business 2nd Edition Debra Johnson Colin Turner p. 59 2. Nam-Hoon Kang, Organisation For Economic Co-Operation And Development, 2001 3. Economics For Business 5th Edition John Sloman p. 498, Development In Practice Taylor & Francis p. 524 4. Tourism In The Age Of Globalisation by Salah Wahab, Chris Cooper (Bradley 1993, Dicken 1992) p. 320-323 5. Economics By Richard G. Lipsey, K. Alec Chrystal p. 11 6. ELECTRONIC COMMERCE AND THE GLOBALISATION ERA by STUPARU, DRAGOS, VASILE, TOMITA (article) 7. Economics of globalisation By Partha Gangopadhyay, Manas Chatterji p. 191-193 8.Globalisation: prospects and policy responses, fourteenth report of session †¦ By Great Britain: Parliament: House of Commons: Treasury Committee p. 7-12 9. http://www. globaldesignandbusiness. org 10. http://www. wto. org 11. http://www. nafta-sec-alena. org 12. Global public policy: governing without government: Wolfgang H. Reinicke p. 15 13. Privatization in transition countries: By Oleh Havrylyshyn, Donal McGettigan p. 7-8 14. Monetary Policy under Uncertainty Proceedings of the 2007 Money and Banking Seminar – BCRA p. 86 (report) 15. http://www. aseansec. org 16. http://europa. eu 17. http://en. wikipedia. org